You are here
Dynamics of reproduction of vermiculture in industrial conditions under the influence of Humilid
The article dedicates to the results of the study of impact of Humilid on the reproduction of the red Californian worm and the frequency of occurrence of vermiculture with different weights in the process of vermicultivation for 6 months.
The stadies were conducted under conditions of vermifarm of “Natural Biotechnology” (Zaporizhzhya), which is a producer of vermicompost, liquid humic drug and biomass of vermiculture. The object of research was a hybrid of red Californian worm. The nutrient substrate was a mixture of fermented manure of cattle and fermented sunflower husk (wastes of mashroom production) in the ratio of 9: 1. Clamps formed 5х0,5х0,15 m size and they were sown with vermiculture in the amount of 5-7 thousand average per 1 m2. Fresh substrate was distributed by layer of 7-10 cm across surface of the clamp. Once every 7-10 days it was watered. The room temperature maintained in the range of 21-24 0С, substrate humidity was the in the range of 65-78 %, which corresponds to the technological conditions of cultivation. Research clamps differed from control clamps by the presence of biologically additive "Humilid"[TU 15.7-00493675-004:2009] in an amount of 15 mg / kg of nutrient substrate, which was contributed once per month. Vermicultivation performed within 6 months.
There were taken spot samples of the vermiculture in the clamps with intervals of 45 days, of which were preparing medium samples and were recalculating their number per 1 m2 (square meter).On the 135th and the 180th days of our research there were defined the mass of the red hybrid Californian worm from the medium sample. The statistical calculation has been done with the help of the editor "Microsoft Excel".
At the beginning of our research work in control and research clamps in average were settled 7 thousand worms per 1 m2 . On the 45th day of the experiment the amount of worms has grown up on 13,9 % (р<0,01) in relation to control in the clamps, where were put biologically active feed addition of “Humilid”. Their number has increased by 2,1 times in the control group, and in the research group it has increased by 2,4 times in comparison with the beginning of our research. On the 90th day of the research the number of vermiculture beings has increased on 16,1 % (р<0,001) in the group, where we put Humilid to the nutritional substrate in relation to the control one. In comparison with the beginning of our research the number of the red hybrid Californian worm has increased by 3,4 times in the control group, and it has increased by 5,0 times in the research group. On the 135th day of the research Humilid in mixture with the nutritional substrate facilitated to the increase of the number of vermiculture beings on 19,7 % (р<0,001) in comparison with the control. At the end of the research (180 day) the number of vermiculture has increased on 21,1 % (р<0,001) in the group with the addition of “Humilid” in comparison with the control. At the same time the number of the red hybrid Californian worm in the research and in the control groups has increased by 16,9 and 20,7 times accordingly with the beginning of the research. Thus, the adding Humilid to the nutritional substrate has facilitated to the increase of the number of vermiculture beings. Possibly, such increase is connected with the growth and the development of the red Californian worm, and also with the activation of its reproductive function.
Besides, the research of the frequency of the occurrence of vermiculture with different mass has showed us that on the 135th day of the research in the control and the research groups the frequency of the occurrence of vermiculture with masses 0-0,20, 0,21-0,40, 0,41-0,60, 0,61-0,80 accordingly almost weren’t different. Concerning the end of the experiment the biggest number of the red Californian worm was 64,7 % with the middle mass 0,21-0,40 g from the total number of vermiculture in the research group. We consider, that it is connected with the growth of the number of the red hybrid Californian worm, that is the growth of the population density per 1m2 . Probably, the increase of the number of vermiculture beings in the research group is connected with the decrease of heavy metals in biomass of the red Californian worm under the influence of Humilid. Such growth of vermiculture can cause at the same time the bigger accumulation humic substances in biohumus in comparison with the control variant.
It has been found that Humilid in mixture with the nutritional substrate facilitated to the increase of the number of vermiculture beings on 21,1 % (р<0,001) according to the control at the end of the observation.
At the beginning of the observation Humilid doesn’t influence the division of vermiculture according to its mass. At the end of research the number of vermiculture beings with medium mass increases by 1,4 times in comparison with the control one.
Key words: biomass, vermiculture, Humilide, occurrence, accumulation, average mass.
1. Edwards, A., Norman, Q. Arancon, Rhonda, L. (2010) Sherman Vermiculture Technology: Earthworms, Organic Wastes, and Environmental Management / [edited by Clive ], Fla. : CRC Press, p. 623, [1] c.
2. Vovkogon, A. G., Merzlov, S. V. (2014) Efektyvnist' zastosuvannja zbagachenoi' Jodom biomasy vermykul'tury u skladi kombikormiv dlja kurchat-brojleriv [Tekst], [Efficacy of enriched Iodine biomasy of vermiculture as part of feed for broiler chickens]. Naukovo-vyrobnychyj zhurnal «Suchasne ptahivnyctvo», [Scientific-Production magazine «Modern Poultry»], no. 7(140), pp. 8-10
3. Irikov O.V., Zabudsky, Yu. I. Pat. 2470521 (2011) Rossijskaja Federacija, MPK A23K 1/100. Sposob proizvodstva belkovo-vitaminnoj kormovoj muki iz gibrida krasnogo kalifornijskogo dozhdevogo chervja i vermikompostirovannyh jablochnyh vyzhimok [Tekst], [Russian Federation, IPC А23К 1/100. Method of production of protein-vitamin fodder flour from a hybrid of a red Californian earthworm and vermicomposted apple pimples, Bjul. no. 36.
4. Stepchenko, L. M., Gejsun, A. A., Galuzina. L. I, (2017) Efektyvnist' zastosuvannja biomasy vermykul'tury, shho otrymana z vykorystannjam Gumilidu u godivli molodnjaku fazanu myslyvs'kogo [Tekst], [Efficiency of use of biomass of vermiculture, with has been received with the use of Нumilid in the feeding of the young pheasant hunting]. Problemy zooinzhenerii' ta veterynarnoi' medycyny. Zbirnyk naukovyh prac' Harkivs'koi' derzhavnoi' zooveterynarnoi' akademii, Problems of zoinengineering and veterinary medicine Collection of scientific works of Kharkiv State Animal Veterinary Academy]. Vyp 34, Ch 2, pp. 105-109.
5. Slobodian ,V. O., Slobodian, N. S. Pat. 35923 (1999) Ukrai'na, MPK (2006.01) 6 A61K35/56. Sposib oderzhannja biologichno aktyvnyh frakcij z doshhovyh cherv'jakiv [Tekst],[ Method of obtaining biologically active fractions from earthworms], Ukrai'na), № 99041932, Bjul.no. 3.
6. Petrov, V.F; Safonova, G.M; Hilko, M.G.; Zakirov, M.R. Pat. 2177784 (1999) Rossijskaja Federacija, MPK A61K9/20, A61K35/56. Jenteral'nyj preparat, soderzhashhij jekstrakt biomassy krasnogo kalifornijskogo dozhdevogo chervja Eisenia foetida v vide tabletok "Vermin" [Tekst], [Enteral preparation containing the extract of the biomass of the red Californian earthworm Eisenia foetida in the form of tablets "Vermin"] patentoobladatel' Permskoe nauchno-proizvodstvennoe ob#edinenie "Biomed", no. zajavki 99111405/14.
7. Petrov, VF, Safonova, GM, Perevozchikova, EN, Korosteleva, LK Pat. 2180574 (1999) Rossijskaja Federacija, MPK A61K35/56, A61R31/00. Maz' dlja lechenija inficirovannyh ran [Tekst], [Ointment for the treatment of infected wounds], patentoobladatel' Permskoe nauchno-proizvodstvennoe ob#edinenie "Biomed",no. 99106745/14.
8. Shikula, M.K., Dulnev, P.G., Fantuch, V.S.Pat. 35923 (2003) Ukrai'na, MPK7 C07D 213/89, S07S 55/06, A01K 67/06. Zasib dlja zbil'shennja reproduktyvnosti cherv'jakiv ta sposib jogo oderzhannja. [Tekst], [A tool to increase the reproductive capacity of worms and how to get it], no. 54507, Bjul.no. 3.
9. Stom, D. I., Antonova, E.V. (2001) Jekstragirovanie kokonov dozhdevyh chervej [Tekst], [Extracting cocoons of earthworms].Bioraznoobrazie Bajkal'skogo regiona, [Biodiversity of the Baikal region. – Irkutsk], pp. 2–8.
10. Vovkogon, A. G., Merzlov, S.V. (2014) Vplyv riznyh dzherel ta doz jodu na naroshhuvannja biomasy gibryda chervonyh kalifornijs'kyh cherv'jakiv [Tekst], [Influence of various sources and doses of iodine on the growth of biomass of the hybrid of red Californian worms].Naukovyj visnyk NUBIP Ukrai'ny. Serija: Tehnologija vyrobnyctva i pererobky produkcii' tvarynnyctva, [Scientific Bulletin of NUBIP of Ukraine. Series: Technology of production and processing of livestock products ], no. 202, pp. 286– 291.
11. Merzlov, S. V., Mashkin, Yu.O. (2015) Naroshhuvannja biomasy cherv'jakiv za riznyh koncentracij ferumu v substrati [Tekst], [Arrangement of worm biomass at different concentrations of the ferrum in the substrate]. Tehnologija vyrobnyctva i pererobky produkcii' tvarynnyctva, [Technology of production and processing of livestock products], no.1, pp. 103– 106.
12. Gejsun, A. A., Stepchenko, L. M. (2016) Doslidzhennja rostu ta rozvytku vermykul'tury za vplyvu Gumilidu [Tekst], [Investigation of growth and development of vermiculture under the influence of Нumilid]. Tehnologija vyrobnyctva i pererobky produkcii' tvarynnyctva. Naukovyj visnyk NUBiP Ukrai'ny, [Technology of production and processing of livestock products. Science Herald NUBіP Ukraine], Vyp. 236, pp. 316 – 325.
13. Myhajlenko, Je.O., Dumshina, O.O., Ushakova, G.O., Stepchenko, L.M. (2016) Efektyvnist' antyoksydantnoi' systemy pechinky brojleriv krosu Kobb-50 pry vypojuvanni pryrodnymy biologichno aktyvnymy dobavkamy na osnovi guminovyh rechovyn, [Effectiveness of the antioxidant system of the liver of broilers Cobb-50 crosses when dispensing with natural biologically active additives based on humic substances]. Visnyk Dnipropetrovs'kogo derzhavnogo agrarno-ekonomichnogo universytetu, [Bulletin of the Dnepropetrovsk State Agrarian and Economic University],no 2, pp. 120-125.
14. Stepchenko, L.M. (2010) Reguljatorni mehanizmy dii' biologichno aktyvnyh rechovyn guminovoi' pryrody na organizm produktyvnoi' ptyci, [Control mechanisms of action of biological active substances of humic nature on organism of productive poultry]. Fiziologichnyj zhurnal, [Physiological magazine], T. 56, no. 2, 306 p.
15. Gejsun, A. A., Stepchenko, L. M. (2016). Doslidzhennja vplyvu Gumilidu na kontaminaciju vazhkymy metalamy produktiv vermytehnologii' [Tekst], [Study of Humilid impact on contamination of vermitechnology products by heavy metals]. Tehnologija vyrobnyctva i pererobky produkcii' tvarynnyctva. Zbirnyk naukovyh prac' Bilocerkivs'kogo nacional'nogo agrarnogo universytetu, [Technology of production and processing of livestock products. Collection of scientific works of Bila Tserkva National Agrarian University], Vyp 2 (129), pp. 68-74.
16. Gejsun, A. A. (20170 Vplyvu Gumilidu na nakopychennja guminovyh rechovyn u biogumusi [Tekst], [The influence of Humilid on the accumulation of humin substances in a biohumus]. Visnyk Dnipropetrovs'kogo derzhavnogo agrarno-ekonomichnogo universytetu, [Bulletin of the Dnipropetrovsk State Agrarian-Economic University], no. 1(43), pp. 17-20
| Attachment | Size |
|---|---|
 geisun_btf_1-2_2017.pdf | 6 MB |