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Productivity and biochemical indices in broiler chickens liver under enzyme preparations influence

The research was conducted in Bila Tserkva National Agrarian University vivarium. 5 groups of broiler chickens (one control and four experimental ones), 100 chicks each, were formed for the experiment. The chickens were kept on deep litter.

The control group birds were fed with full ration animal feed without additional cellulase enzyme additive inclusion. First control group birds were fed with the feed including 0.1 g/kg cellulose obtained with Aspergillus terreus strain cultivated on the nutritious substance without additional Cuprum adding. The II, III and IV research groups received respectively 0,068; 0,061 and 0,054 g/kg cellulose enzyme obtained with Aspergillus terreus strain cultivated on the nutritious substance with optimal Cuprum chelate dose adding.

The enzyme supplements were produced at "BTU Center" laboratory of Ladyzhin town, Vinnytsia region. At the end of the experiment we conducted the poultry performance records and it’s slaughter. The slaughtered chicken liver were taken for biochemical studies on the enzymes activity measure.

Analysis of the research results obtained with the improved biotechnology shoes that using cellulase in feeding broiler chickens results in their growth increase and weight accumulating.

At the beginning of the experiment control and experimental groups chickens hardly differed by their live weight. In subsequent age periods (11, 21, 31 and 42 days) the chickens live weight varied depending on the level of cellulase activity in the fodder.

It is proved that at the end of the experiment, average live weight of broilers was higher in all experimental groups compared with the control. Feeding 0,068 g / kg cellulase in the second experimental group contributed to the increase of live weight poultry by 9.5% (p≤0,001) relative to the control.

The increase of broiler live weight in the experimental groups can be explained by the fact that under the action of cellulases a significant amount of cellulose is hydrolyzed and hydrolyzed cellulose is an antinourishing factor for the poultry, which subsequently affects positively the growth of the of nutrients transformation into the products.

The poultry productivity increase under the influence of the enzyme aminotransferases growth is confirmed by the increased activity and a probable increase in protein content in the chickens liver, the rate was higher than the control, respectively, by 17.5 and 22.1 %.

At the end of the experiment conducted performance records of birds and their slaughter. From slaughtered chicken liver were taken for biochemical studies, which measured enzyme activity. Analyzing the results of the study of the impact of biotechnology cellulase improved the performance of the birds found that the use in feeding broiler feed new factor is the increased growth and accumulation of mass.

At the beginning of the experiment and the control chickens research groups hardly differed by live weight. In later ages (11, 21, 31 and 42 days) live weight of chickens varied depending on fluctuations cellulase activity levels in the diet.

It is proved that at the end of the experiment, average live weight of broiler chickens was higher in all experimental groups compared to the control. Insertion of cellulase 0.068 g / kg feed in the second experimental group has contributed to the live weight of poultry by 9.5% (p≤0,001) relative to controls.

The increase in live weight broiler in experimental groups can be explained by the fact that under the action of cellulases hydrolyzed significant amount of pulp that is antypozhyvnym factor for the birds, and this in turn positively affects the growth transformation of nutrients in feed products.

Aspartic (AsT) and alanine transferase (AlT) are localized in the cells of most organs and systems. They carry the amino group of aspartic acid and alanine in alfaketohlutarovu acid. Alkaline phosphatase some extent characterizes the state of phosphorus-calcium metabolism in tissues and direct exchange of organophosphorus compounds. Alkaline phosphatase is a zinc metallo-protein, which is involved in mineral metabolism.

broiler chickens II experimental group activity aspartate aminotransferase was higher than in controls to 11.3 %. Increased activity of AST in the liver of birds I, III and IV research groups relative to the control was determined at trends. Just a little character growth trends alanine aminotransferase activity in the liver of all experimental groups. Thus, increased activity of alanine and aspartataminotransferases may indicate a strengthening of biosynthetic processes in the body broiler chickens due to their intense growth and confirms the impact of cellulolytic enzymes in protein metabolism in animals.

Feeding the research groups poultry the fodder containing cellulolytic enzyme produced under the advanced biotechnology was not accompanied by changes in the activity of alkaline phosphatase in the liver of broiler chickens, the indicators were at the control level.

No changes in the activity of alkaline phosphatase in the liver of experimental groups broiler chickens compared with the control, indicate absence of organic substances dephosphorylising processes fails and metabolic acids active transporting.

Catalase belongs to the enzymes containing Ferum and thus catalase activity can be used to judge on poultry body antioxidant status.

The activity of catalase in the experimental group and control varied from 9.0 %, but the difference was unlikely.

Key words: live weight, performance, broiler chickens, protein, cellulases, aspartataminotransferase, alaninaminotrans-
ferase, alkaline phosphatase, catalase.

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