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EFFECT OF PROLACTIN GENE POLYMORPHISM ON MILK PRODUCTION TRAITS IN UKRAINIAN BLACK-AND-WHITE DAIRY CATTLE
The objective of this study was to elucidate the effect of prolactin (PRL) gene polymorphism on milk production traits involving total milk yield, milk composition and technological properties in Ukrainian Black-and-White dairy cattle (n=200). PCR-RFLP method was used to PRL genotypes detecting. In this herd, the frequencies of alleles were follows: A=0,120 and G=0,880. The frequencies of AA, AG and GG genotypes were 0,017, 0,211 and 0,772 respectively.
Results showed that PRL AA genotype had a statistically significant (P<0,05) higher milk yield and milk protein yield in comparison with alternative genotypes. With respect to technological properties (milk rennetability and heat stability), the AA genotype had higher levels than the AG and GG individuals. The obtained data confirmed that PRL may be a strong candidate gene for application in marker assisted selection and could be used as an important source of information for producers as well as for breeders.
Key words: milk production traits, prolactin gene, PCR-RFLP, genotypes, milk yield, milk composition, rennetability, heat stability.
1. Gareeva I.T. Vzaimosvjaz' polimorfnyh variantov genov prolaktina i β-laktoglobulina s molochnoj produktivnost'ju korov: avtoref dis. na soiskanie uchenoj stepeni kand. biol. nauk: 06.02.07. – razvedenie, selekcija i genetika s.-h. zhivotnyh / I.T. Gareeva. – Sankt-Peterbyrg-Pushkin, 2012. – 22 s.
2. Kugenev P.V. Praktikum po molochnomu delu / P.V. Kugenev, N.V. Barabanshhikov. – M.: Agropromizdat, 1988. – 224 s.
3. Novak N.B. Analіz genetichnoї strukturi VRH ta bіotehnologіchnі pіdhodi shhodo vdoskonalennja pokaznikіv molochnoї produktivnostі / N.B. Novak, R.V. Oblap // Vіsnik Sums'kogo nacіonal'nogo agrarnogo unіversitetu, Ser. Tvarinnictvo. – 2012. – Vip. 12 (21). – S. 73–75.
4. Metodichnі rekomendacії shhodo vikoristannja metodu polіmeraznoї lancjugovoї reakcії v skotarstvі / [Oblap R.V., Novak N.B., Mel'nichuk M.D. ta іn.]; za red. T.M. Diman'. – Bіla Cerkva, 2010. – 66 s.
5. Alipanah M. Association of prolactin gene variants with milk production traits in Russian Red Pied cattle / M. Alipanah, L. Kalashnikova, G. Rodionov // Iran. J. Biotechnol. –2007. – Vol. 5 (3). – P. 158–161.
6. Brym P. Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits / P. Brym, S. Kamiñski, E. Wójcik // J. Appl. Genet. – 2005. – Vol. 45 (2). – P.179–185.
7. Dahl G.E. Effects of short day photoperiod on prolactin signaling in dry cows: a common mechanism among tissues and environments? / G.E. Dahl // J. Anim. Sci. – 2008. – Vol. 86. – P. 10–14.
8. Dybus A. Association of genetic variants of bovine prolactin with milk production traits of Black-and-White and Jersey cattle / A. Dybus, W. Grzesiak, H. Kamieniecki // Arch. Tierz. – 2005. – Vol. 48. – P. 149–156.
9. Khatami S.R. Association of DNA polymorphisms of the growth hormone and prolactin genes with milk productivity in Yaroslavl and black-and white cattle / S.R. Khatami, O.E. Lazebnyi, V.F. Maksimenko // Russ. J. Genet. – 2005. – Vol. 41 (2). – P. 229–236.
10. Michalova A. Influence of composite k-casein and β-lactoglobulin genotypes on composition, rennetability and heat stability of milk of cows of Slovak Pied breed / A. Michalova, Z. Krupova // Czech J. Anim. Sci. – 2007. – Vol. 52 (9). –
P. 292–298.
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