You are here

Головна » BTF №2 2022

Features of herd formation based on betaand kappa-casein of different dairy cattle breeds

In dairy cattle breeding, much attention is paid to indicators of the milk quality and technological properties. These indicators are significantly influenced by milk proteins. Therefore, the goal of our research is to evaluate the effectiveness of creating dairy herds of domestic dairy cattle breeds that are characterized by the desired beta- and kappa-casein genotypes. To fulfill these tasks, the genotyping of cows of the Ukrainian Brown dairy, Sumy intrabreed type of the Ukrainian Black-and-White dairy, Simmental and Lebedyn breeds was carried out. The beta- and kappa-casein gene polymorphism was determined in the genetic laboratory of the Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine using real-time molecular biological analysis of allele recognition by polymerase chain reaction (PCR). As a result of the conducted studies, it is found that cattle of Lebedyn and Ukrainian Brown dairy breeds have a higher frequency of the desired kappa-casein BB genotype. As a result, they are also characterized by a higher frequency of the B allele. More than 50 % of cattle of Simmental breed have a heterozygous AB genotype. Cattle of the Sumy intrabreed type of the Ukrainian Black-and-White dairy breed have a higher frequency of the homozygous AA genotype and the A allele. Cattle of the Ukrainian Brown dairy breed are characterized by the highest frequency of the A2A2 genotype and the A2 allele of beta-casein. Cattle of the Simmental breed, which are also characterized by the highest frequency of the A1A2 heterozygous genotype, have a slightly lower frequency. Homozygous A1A1genotype is more common in cattle of the Sumy intrabreed type of the Ukrainian Black-and-White dairy breed. Using the principles of the model for creating a herd with the desired genotype, which is proposed by Mencarini IR et all, it is found that when testing the entire breeding stock, inseminating it with semen of homozygous stud bulls with the A2A2 genotype, random cow disposal at the level of 22 %, it takes 9–10 years to create herds with the desired genotypes of the Ukrainian Brown dairy, Simmental and Lebedyn breeds, respectively. To reduce the period for creating herds, it is essential to increase the percentage of cow disposal and simultaneously disposecattle with undesirable genotypes.

Key words: genotype, casein, stud bull, breeding, allele.

 

 

 

LADYKA V. https://orcid.org/0000-0001-6748-7616


PAVLENKO Y. https://orcid.org/0000-0002-4128-122X


SKLYARENKO Y. https://orcid.org/0000-0002-6579-2382


  1. Anggraenia, A., Sumantrib, C., Farajallahc, A., Andreasd, E. (2010). Kappa-Casein Genotypic Frequencies in Holstein-Friesian Dairy Ca le in West Java Province. Media Peternakan, no. 33 (2), pp. 61–67.
  2. Bezdíček, J. (2007). Allele and genotype frequencies of milkprotein kappa-casein (CSN3) in artifical insemination bulls of Czech fleckvieh and holstein breed. Sborník mendelovy zemědělské a lesnické univerzity v brně. no.5, рp. 17–22.
  3. Bisutti, V., Pegolo, S., Giannuzzi, D., Mota, L., Vanzin, A., Toscano, A., Trevisi, E., Ajmone P. Marsan, M., Brasca, A. Cecchinato. (2022). The β-casein (CSN2) A2 allelic variant alters milk protein profile and slightly worsens coagulation properties in Holstein cows. Journal of Dairy Science, no. 105 (5).
  4. Botaro, B., Vinícius, Y., Simões, C. (2009). Effect of the kappa-casein gene polymorphism, breed and seasonality on physicochemical characteristics, composition and stability of bovine milk. Revista Brasileira de Zootecnia. no. 38 (12), pp. 2447–2454.
  5. Deb, R., Singh, U., Kumar, S., Singh, R., Sengar, G., Sharma, A. (2014). Genetic polymorphism and association of kappa-casein gene with milk production traits among Frieswal (HF × Sahiwal) cross breed of Indian origin. Journal of Veterinary Research, Shiraz University IJVR, no. 15 (4), pp. 406–408.
  6. Gallinat, J., Qanbari, S., Drögemüller, C., Pimentel, E., Thaller, G., Tetens, J. (2013). DNA-based identification of novel bovine casein gene variants. J. Dairy Sci., no. 96 (1), pp. 699–709.
  7. Giribaldi, M. Lamberti, C., Cirrincione, S.,Giuffrida, M.G., Cavallarin, L. (2022). A2 Milkand BCM-7 Peptideas Emerging Parameters of Milk Quality. Front. Nutr. no. 9.
  8. Gustavsson, F., Buitenhuis, A., Johansson, M., Bertelsen, H., Glantz, M., Poulsen, N. (2013). Effects of breed and casein genetic variants on protein profile in milk from Swedish Red, Danish Holstein, and Danish Jersey cows. J. Dairy Sci., no. 97, pp. 3866–3877.
  9. Klauzinska, M., Siadkowska, E., Grochowska, R. (2001). Polymorphism of molecular-genetic systems in the Polish red cattle. Tsitol Genet. no. 35 (1), pp. 58–60. DOI:10.31080/ASVS. 2022.04.0429
  10. Ladyka, V., Drevytska T., Pavlenko J., Skliarenko Y., Lahuta T., Drevytskyi O., Dosenko V. (2022). Evaluation of cow genotypes by kappa-casein of dairy breeds. Acta fytotechn zootechn. no. 25,(1) pp. 1–6. (in Ukrainian) Ladyka, V., Pavlenko, Y. Sklyarenko Y. (2021). Usodelpolimorfismodel gen de la β-caseína en términos de preservación del ganado lechero marrón. Arch. Zootec. no. 70(269), pp. 88–94. (in Ukraine)
  11. Lateef, Y.M., Hamad, R. (2019). Effect of mutation site of k-casein gene on protein quantity, composition, and other milk constituents in Holstein cows. J. Pharm. Sci., no. 11(2), pp. 398–401.
  12. Leveziel, H., Metenier, L., Mahe, M. (1988). Identification of the two common alleles of the bovine k-casein locus by the RFLP technicue, using the enzyme Hind III. Genet. Sel. Evol. no. 20, 247 p.
  13. Mencarini, I.R., Woodford, K.B., Old, K.M. (2013). Comparing herd selection strategies for A2 beta-casein. Proceedings of the NewZealand Society of Animal Production. no. 73, pp.149–154.
  14. Poulsen, N., Glantz, M., Rosengaard, N., Paulsson, M., Larsen, L. (2017). Comparison of milk protein composition and rennet coagulation properties in native Swedish dairy cow breeds and high-yielding Swedish Red cows. J. Dairy Sci., no. 100, pp. 8722– 8734.
  15. Reddy, B., Lokavya, R. (2022). The Science of A1 & A2 Milk: Decoding the Enigma. Acta Scientific Veterinary Sciences. no. 4.7, pp. 54–60.
  16. Sebastiani, C., Arcangeli, C., Torricelli, M., Ciullo, M., D’avino, N., Cinti, G., Fisichella, S., Biagetti, M. (2022). Marker-assisted selection of dairy cows for β-casein gene A2 variant. Italian Journal of Food Science, 34 (2), pp. 21–27.
  17. Vigolo, V., Franzoi, M., Cendron, F., Salvadore, G., Penasa, M., Cassandro, M., Marchi, M.De. (2022). Characterization of the genetic polymorphism linked to the β-casein A1/A2 alleles using different molecular and iochemical methods. Journal of Dairy Science, no. 105 (11).
  18. Vitte, K., Kerziene, S., Klementavičiūtė, J., Vitte, M., Mišeikienė, R., Kudlinskienė, L., Čepaitė, J., Dilbiene, V., Stankevičius, R. (2022). Relationship of β-casein genotypes (A1A1, A1A2 and A2A2) to the physicochemical composition and sensory characteristics of cows’ milk. Journal of Applied Animal Research, no. 50, 1, pp.161–166.
  19. Zepeda-Batista, J., Saavedra-Jiménez, A., Ruíz-Flores, А., Núñez-Domínguez, R., RamírezValverde, L. (2017). Potential influence of κ-casein and β-lactoglobulin genes in genetic association studies of milk quality traits. Asian-Australasian Journal of Animal Sciences, no. 30 (12), pp. 1684–1688.

 

 

 

 

AttachmentSize
PDF icon ladyka_2_2022.pdf443.17 KB
BTF №2 2022