You are here

Головна » 2021 » BTF №1 2021

The impact assessment of the drug "Artificial cuticle" on the development of chicken embryos

The article presents the results of experimental research as for the possible toxic effects of the disinfectant "Artificial cuticle" ("ARTICLE" – ARTIficial cutiCLE) which includes a substance of natural origin chitosan acid-soluble, peracetic acid, yellow iron oxide pigment (oxide II) Fe2O3 and microelements. This pre-incubation treatment technology involves irrigating the eggs with a working solution of the composition, followed by evaporation of the solvent and the formation on the surface of the calcite eggs’ layer an exact artificial analogue of the natural protective cuticle. Barrier properties of bioceramic structures of the shell and shell membranes are characterized by biocidal (antibacterial and antiviral) activity, as well as the ability to optimize embryo gas exchange during incubation and improve embryo metabolism and the quality of young birds. The studies were performed using hatching eggs obtained from laying hens Leghorn white. Two batches of 720 eggs were formed in each: control eggs were treated with formaldehyde fumigation, the second batch was treated with the drug "Artificial Cuticle". Incubation of eggs was performed in an incubator "Universal" for 21 days according to the method. On the 11th day of incubation, chicken embryos were removed, tissue and liver preparations were prepared. Prepared histological specimens were viewed under a microscope. Under the influence of the disinfectant on the chicken embryo, no abnormalities in the development of the embryo and the morphological structure of the liver were detected in comparison with the control. Similar to the control of the corresponding observation period, the formed liver beams, the beginning of the formation of bile and Kupffer cells are detected. Thus, when comparing control and experimental samples of embryonic liver tissue, the negative impact of the disinfectant "Artificial Cuticle" was not detected. The components of the drug do not enter the area of embryonic development. The drug "Artificial Cuticle" has no toxic effect on the developing embryo during incubation.

Key words: technology, chitosan, nanotechnologies, egg incubation, disinfectants, histological examinations.

BORDUNOVA O. http://orcid.org/:0000-0002-7120-1040


ASTRAHANTSEVA O. http://orcid.org/:0000-0002-2624-837


PETRENKO H. http://orcid.org/:0000-0002-2624-8376


DOLBANOSOVA R. https://orcid.org/0000-0002-3047-7067


  1. Astrakhantseva, O.H., Samokhina, Ye.A., Bordunova, O.H., Chivanov, V. D. (2016). Kompozytsiia dlia zakhystu inkubatsiinykh yaiets kurei na osnovi khitozanu, perekysnykh spoluk i sulfatu zaliza (FeSO4 5H2O) [Composition for protection of hatching eggs of chickens based on chitosan, peroxide compounds and ferrous sulfate (FeSO4 5H2O)]. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu [Bulletin of Sumy National Agrarian University]. "Livestock". Sumy: SNAU, (7), pp. 207–213.
  2. Breslavets, V. (2001). Inkubacija: metodychnyj posibnyk [ncubation: methodical manual]. Kharkiv: JV UAAS, pp. 42–56.
  3. Ayman, S. Elmezayyen., Fikry, M. Reicha. (2015). Preparation of Chitosan copper complexes: Molecular dynamic studies of Chitosan and Chitosan copper complexes. Open Journal of Applied Sciences. Vol. 5, no. 8, 12 p. Available at:https://doi.org/10.4236/ojapps.2015.58041.
  4. Sargheini, A. (2012). «One-step facile synthesis of CaCO3 nanoparticles via mechano-chemical route». Powder Technology. Vol. 219, pp. 72–77.
  5. Bordunova, O.G., Loboda, V.B., Samokhina, Y.A., Chernenko, O.M., Dolbanosova, R.V., Chivanov, V.D. (2020). Study of the Correlations Between the Dynamics of Thermal Destruction and the Morphological Parameters of Biogenic Calcites by the Method of Thermoprogrammed Desorption Mass Spectrometry (TPD-MS). Springer Proceedings in Physics. Springer, Singapore, Vol 240, pp. 37–50. Available at:https://doi.org/10.1007/978-981-15-1742-6
  6. Cenzig, Caner., Ozge, Cansiz. (2007). Chitosan coating minimises eggshell breakage and improves egg quality. Journal of the Science of Food and Agriculture. Vol. 88, Issue 1, pp. 56–61.
  7. Jones, D. R. (2018). Impact of egg handling and conditions during extended storage on egg quality. Poultry science. Issue 97(2), pp.716–723.
  8. D’Alba, L. «Antimicrobial properties of a nanostructured eggshell from a compostnesting bird». J. Exp Biol. Vol. 217.- pp.1116–1121, 2014.
  9. Leleu, S., Messens, W., De Reu, K., De Preter, S., Herman, L., Heyndrickx, M., Bain, M. (2011). Effect of egg washing on the cuticle quality of brown and white table eggs. Journal of Food Protection. Issue 74(10), pp. 1649–1654.
  10. Liu, Y.C., Chen, T.H., Wu, Y.C., Lee, Y.C., Tan, F.J. (2016). Effects of egg washing and storage temperature on the quality of eggshell cuticle and eggs. Food Chemistry. Issue 211, pp. 687–693.
  11. Gritsch, L., Lovell, Ch., Wolfgang, H. Goldmann., Aldo, R. Boccaccini. (2018). Fabrication and characterization of copper(II)-chitosan complexes as antibiotic-free antibacterial biomaterial. Carbohydrate Polymers. Vol. 179, pp. 370–378. Available at:https://doi.org/10.1016/j. carbpol.2017.09.095.
  12. Bordunova O. (2012). «Method of protection of hatching eggs of chickens with chitosan coating». Patent № 59917 (Ukraine).
  13. Wellman-Labadie, O., Picman, J. Hincke, M.T. (2008). «Antimicrobial activity of cuticle and outer eggshellprotein extracts from three species of domestic birds». British Poultry Science. Vol. 49(2), pp. 133–143.
  14. Bhadauria, P. (2014). «Impact of Hot Climate on Poultry Production System-A Review». Journal of Poultry Science and Technology. Vol. 2(4), pp. 56–63.
  15. Ataee, R. A. (2011). «Antibacterial effect of calcium carbonate nanoparticles on Agrobacterium tumefaciens». Iranian Journal of Military Medicine. Vol. 13 (2), pp. 65–70.
  16. Samiullah, K., Chousalkar, K. K., Roberts, J. R., Sexton, M., May, D., Kiermeier, A. (2013). Effects of eggs shell quality and washing on Salmonella Infantis penetration. International Journal of Food Microbiology. Issue 165(2), pp. 77–83.
  17. Xian, De. (2009). Effect of combination of chitosan coating and irradiation on physicochemical and functional properties of chicken egg during room-temperature storage. Radiation Physics and Chemistry. Vol. 78, Issues 7-8, pp. 589–591.
  18. Wojtasz-Pajak, A., Balicka-Ramisz, A., Ramisz, A., Ligocki, M. (2003). Properties of chitosan and its salt. In: Chemical Products in Agriculture and Enviroment/ Eds. E. H. Gorecki, Z. Dobrzanski. Chech-Pol Trade (Prague, Brussels, Stockholm). pp. 74–80.
AttachmentSize
PDF icon bordunova_1_2021.pdf700.59 KB
2021
BTF №1 2021