You are here

Головна » BTF №2 2023

Natural resistance and immunological response of young Volyn meat breed to the action of probiotics

To investigate the response of natural resistance and immunological reactions of newborn young animals of the Volyn beef breed to the use of probiotic preparations. To conduct experimental research, three groups were formed from young Volyn meat breeds: the control group was fed only on mother's milk, the first group received a probiotic preparation with Bacillus Subtilis strain in addition to cow's milk, the second experimental group –– Lactobacillus spp. The activity of natural resistance and the body's immune response were studied on the 5th, 10th, and 30th day of the experiment. The level of nonspecific natural resistance was determined by indicators of bactericidal, lysozyme, phagocytic, and complementary activity of blood serum, and the immunological response was determined by the concentration of immunoglobulins of classes G, M, and A in the blood. It was established that starting from the 5th day of life, a difference in the nonspecific resistance of control and experimental animals was noted groups In separate age periods, probiotic supplements had a different degree of influence on the natural resistance of young animals. The greatest difference in the direction of growth in such indicators of non-specific resistance as bactericidal and complementary activity of blood serum was noted under the influence of the probiotic supplement Bacillus Subtilis (I experimental group), and indicators of lysozyme and phagocytic activity of blood serum were greater under the action of Lactobacillus spp. (II experimental group). The difference between indicators of bactericidal activity of blood under the action of Lactobacillus spp. in comparison with the control was at the age of 30 days (16.85 %, Р<0.01), and under the action of Bacillus Subtilis –– at the age of 60 days (25.49 %, Р<0.001). The level of phagocytic and complementary blood activity was the highest at the age of 60 days in the I experimental group –– by 17.17 % (Р<0.001) and 32.57 % (Р<0.001), in the II experimental group –– by 23.20 % (Р <0.001) and 36.34 % (Р<0.001), respectively. Regarding the concentration of immunoglobulins, the largest changes in their indicators were noted in the group that received Lactobacillus spp. Addition of probiotic strains of bacteria to the diet of young animals has a positive effect on the natural resistance and immune response of the body, which in the future will contribute to reducing the level of morbidity in young animals.

Key words: calves, probiotics, bacterial strains, microorganisms, immunoglobulins, non-specific resistance of the organism, T-lymphocytes, phagocytic activity, bactericidal activity of blood serum.

 

FARAFONOV S. https://orcid.org/0000-0002-0000-2562


BORSHCHENKO V. https://orcid.org/0000-0002-0710-5628


STAKHIV V. https://orcid.org/0000-0002-8393-1120


MYLOSTУVA D. https://orcid.org/0000-0002-3609-776X


MYLOSTYVYI R. https://orcid.org/0000-0002-4450-8813


  1. Abuelo, A., Cullens, F., Hanes, A. (2021). Impact of 2 Versus 1 Colostrum Meals on Failure of Transfer of Passive Immunity, Pre-Weaning Morbidity and Mortality, and Performance of Dairy Calves in a Large Dairy Herd. Animals (Basel). Vol. 11, Issue 3, 782 p. DOI:10.3390/ani11030782.
  2. Ahmann, J., Steinhoff-Wagner, J., Büscher, W. (2021). Determining Immunoglobulin Content of Bovine Colostrum and Factors Affecting the Outcome: A Review. Animals (Basel). Vol. 11, Issue 12, 3587 p. DOI:10.3390/ani11123587.
  3. Casewell, M., Friis, C., Marco, E. (2003). The European ban on growth-promoting antibiotics and emerging consequences for human and animal health. J Antimicrob Chemother, Vol. 52, Issue 2, pp.159-161. DOI:10.1093/jac/dkg313.
  4. Cull, C., Singu, V. K., Cull, B. J. (2022). Efficacy of Lactobacillus animalis and Propionibacterium freudenreichii-Based Feed Additives in Reducing Salmonella-Associated Health and Performance Effects in Commercial Beef Calves. Antibiotics (Basel). Vol. 11, Issue 10, 1328 p. DOI:10.3390/ antibiotics11101328.
  5. Dar, A. H., Singh, S. K., Rahman, J. U. (2022). The effects of probiotic Lactobacillus acidophilus and/ or prebiotic mannan oligosaccharides on growth performance, nutrient utilization, blood metabolites, faecal bacteria, and economics of crossbred calves. Iran J Vet Res, Vol. 23, Issue 4, pp. 322-330. DOI: 10.22099/ IJVR.2022.42992.6259.
  6. Fomenky, B. E., Do, D. N., Talbot, G. (2018). Direct-fed microbial supplementation influences the bacteria community composition of the gastrointestinal tract of pre- and post-weaned calves. Sci Rep. Vol. 8, Issue 1, 14147 p. DOI:10.1038/s41598-018-32375-5.
  7. Ghoreishi, S. M., Nouri, M., Rasooli, A. (2015). Effect of orally administered cisapride, bethanechol, and erythromycin on the apparent efficiency of colostral IgG absorption in neonatal Holstein-Friesian calves. J Vet Intern Med., Vol. 29, Issue 2, pp. 714-720. DOI:10.1111/jvim.12539.
  8. Karamzadeh-Dehaghani, A., Towhidi, A., Zhandi, M. (2021). Combined effect of probiotics and specific immunoglobulin Y directed against Escherichia coli on growth performance, diarrhea incidence, and immune system in calves. Animal. Vol. 15, Issue 2, pp. 100-124. DOI:10.1016/j.animal.2020.100124.
  9. Lu, Q., Niu, J., Wu, Y. (2022). Effects of Saccharomyces cerevisiae var. boulardii on growth, incidence of diarrhea, serum immunoglobulins, and rectal microbiota of suckling dairy calves. Livest Sci. Vol. 258, 104875 p. DOI:10.1016/ j.livsci.2022.104875.
  10. Ma, T., Villot, C., Renaud, D. (2020). Linking perturbations to temporal changes in diversity, stability, and compositions of neonatal calf gut microbiota: prediction of diarrhea. ISME J., Vol. 14, Issue 9, pp. 2223-2235. DOI:10.1038/ s41396-020-0678-3.
  11. Martin, P., Vinet, A., Denis, C. (2021). Determination of immunoglobulin concentrations and genetic parameters for colostrum and calf serum in Charolais animals. J Dairy Sci., Vol. 104, Issue 3, pp. 3240-3249. DOI:10.3168/jds.2020-19423.
  12. McGee, M., Earley, B. (2019). Review: passive immunity in beef-suckler calves. Animal. Vol. 13, Issue 4, pp. 810-825. DOI:10.1017/S1751731118003026.
  13. Novak, K. N., Davis, E., Wehnes, C. A. (2012). Effect of supplementation with an electrolyte containing a Bacillus-based direct-fed microbial on immune development in dairy calves. Res Vet Sci. Vol. 92, Issue 3, pp. 427-434. DOI: 10.1016/j.rvsc.2011.04.008.
  14. Quigley, J. D., Hill, T. M., Deikun, L. L. (2017). Effects of amount of colostrum replacer, amount of milk replacer, and housing cleanliness on health, growth, and intake of Holstein calves to 8 weeks of age. J Dairy Sci., Vol. 100, Issue 11, pp. 9177-9185. DOI:10.3168/ jds.2017-12784.
  15. Roodposhti, P. M., Dabiri, N. (2012). Effects of probiotic and prebiotic on average daily gain, fecal shedding of Escherichia coli, and immune system status in newborn female calves. Asian-Australas J Anim Sci., Vol. 25, Issue 9, pp. 1255-1261. DOI:10.5713/ ajas.2011.11312.
  16. Wu, Y., Wang, L., Luo, R. (2021). Effect of a Multispecies Probiotic Mixture on the Growth and Incidence of Diarrhea, Immune Function, and Fecal Microbiota of Pre-weaning Dairy Calves. Front Microbiol. Vol. 14, Issue 12, 681014 p. DOI:10.3389/ fmicb.2021.681014.
  17. Várhidi, Z., Máté, M., Ózsvári, L. (2022). The use of probiotics in nutrition and herd health management in large Hungarian dairy cattle farms. Front Vet Sci. Vol. 9, 957935 p. DOI:10.3389/fvets.2022.957935.
AttachmentSize
PDF icon farafonov_2_2023.pdf496.38 KB
BTF №2 2023