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PHOSPHOLIPIDS CONTENT IN BULL SPERM UNDER THE INFLUENCE OF L-CARNITINE

The changes in the composition of phospholipids in the semen of bulls for the actions of different doses of L-carnitine in their diet were investigated. In  bulls semen there were identified that phospholipids fractions of lizophosphatydylholin, sphingomyelin, phosphatidylinositol, fosfatydyl-serine, phosphatidylcholine, cardiolipin, phosphatidylethanolamine and phosphatidic acid. Adding to feed of L-carnitine causes a change in the ratio between different fractions of phospholipids. The probable increase in the relative content of phosphatidylcholine and phosphatidylethanolamine was established. The content of lizophosphatidylholin in semen of research bulls for the entire study period was significantly decreased, which is probably associated with a decrease in intensity of free radical oxidation of phospholipids. L-carnitine has a positive effect on the metabolism of spermatozoa, stabilizing their structure and increasing the protective capabilities of sperm.

Key words: sperm, bulls, carnitine, sphingomyelin, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, cardiolipin, lysophosphatidylcholine, phosphatidylinositol, phosphatidic acid.

1. Boldyrev A. A. Vvedenie v bioximiyu membran / A.A. Boldyrev. – M.: Vysshaya shkola, 1986. – 109 s.

2. Klimov A.N. Obmen lipidov i lipoproteidov i ego narusheniya / A.N. Klimov, N.G. Nikulcheva. – SPb: Piter Kom, 1999. – 512 s.

3. Klinicheskaya laboratornaya diagnostika v veterinarii. Spravochnoe izdanie / I.P. Kondraxin, N.V. Kurilov, A.G. Mala-xov i dr. – M.: Agropromizdat, 1985. – S. 102–106.

4. Kopelevich V.M. Vitaminopodobnye soedineniya L-karnitin i acetil-L-karnitin: ot bioximicheskix issledovanij k medicinskomu primeneniyu // Ukr. bіoxіm. zhurn., 2005. – 77 (4). – S. 30–50.

5. Yanovich V.G. Obmen lipidov u zhivotnyx v ontogeneze / V.G. Yanovich, P.Z. Lagodyuk. – M. : Agropromizdat, 1991. – 317 s.

6. Abe A. The measurement of lysosomal phospholipase A2 activity in plasma / A. Abe, R. Kelly, J.A. Shaymanа // J. of Lipid Research, 2010. – Vol. 51. – Р. 2464–2470.

7. Role of membrane lipids in developing insulin resistant diabetes mellitus type II in Caucasians and African Americans / H.G. Allen, J.C. Allen, L.C. Boyd, G. Fenner // Nutrition. – 2006, Nov-Dec. – Vol. 22. – S. 1096–1102.

8. Dynamics and function of phospholipase D and phosphatidic acid during phagocytosis / M. Corrotte, S. Chasserot-Golaz, P. Huang et al. // Traffic. – 2006. – № 7. – Р. 365–377.

9. Polyunsaturated fatty acids in plasma lipids of obese children with and without metabolic cardiovascular syndrome / T. Decsi, G. Csabi, K. Torok et al. // Lipids. – 2000. – № 35. – Р. 1179–1184.

10. Folch J. Asimple method for the isolation and purification of total lipids fromanimal tissues / J. Folch, M. Lees, G. Stauley // J. Biol. Chem. – 1957. – Vol. 226. – P. 497.

11. Diacylglycerols and phosphatidates: which molecular species are intracellular messengers? / М. Hodgkin, Т. Pettitt, А. Martin et al. // Biochem. Sci. – 1998. – № 23 (6). – Р. 387–393.

12. Laxalt A. M. Phospholipid signalling in plant defence / A. M. Laxalt, T. Munnik // Curr. Opin. Plant Biol. – 2002. – Vol. 5. – P. 332–338.

13. Nkondjock A. Specific fatty acids and human colorectal cancer: an overview / A. Nkondjock, B. Shatenstein, P. Maisonneuve // Ghadirian Cancer Detect Prev. – 2003. – № 27. – С. 55–62.

14. Shivendra D. Shukla Metabolic turnover of ethanol into cellular lipids and platelet activating factor / D. Shivendra // Alcohol Clin. Exp. Res. – 2001. – Vol. 25, № 5, Suppl ISBRA. – Р. 33–39.

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