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Wing morphometry of worker bees in the central and northern parts of Ukraine

In the flat forest-steppe region of Ukraine, several evolutionary lines of honey bees subspecies Apis mellifera mellifera L., Apis mellifera macedonica Ruttner, and Apis mellifera caucasica Pollmann are believed to be widespread, the territorial boundaries and within-population relationships of which require detailed study.Wing morphometry is one of the available methods for establishing the wing phenotype and morphological features of bees in general. However, the completeness and degree of study of the morphometric features of bee populations present in the territory of Ukraine are insufficient. The aim of the study was to carry out a more accurate and thorough classification of wings, establish morphometric standards of worker bees of local populations of Ukrainian steppe bees, create a methodology for assessing possible hybridization by wing phenotype, and attempt to find bee families with queens suitable for further breeding. To achieve this goal, classical morphometry was used to study the wing phenotype using 8 features: traditional - Ci, Dbi, Disc.sh, Pci, Ri, and additional features proposed by the authors - Сі.2, Сі.3, Сі.2.1, which allowed to increase the level of confidence in the quality of statistical data processing of wing features. Using discriminant analysis of the data and the Statistica software package, a classifier was created and 3717 wings were classified. With sufficient reliability (95.6 %), the wings of 28 bee families were divided into five clusters. Five arrays of standards were formed, two of which probably belong to the population of Ukrainian steppe bees subspecies A. m. macedonica, one to subspecies A. m. Carnica Pollmann, two to hybrids of Ukrainian steppe bees and the Polissya population of subspecies A. m. mellifera. A significant similarity in phenotypes was established between two clusters of investigated wings and wings of bees from the Carpathian region, and the hybridization of worker bees of the indicated territory was determined by the Polissya population of subspecies A. m. mellifera. It is shown how, in the presence of standard data of worker bee wing features obtained as a result of this work and others formed in another, it is possible to use discriminant analysis to correctly classify wings and assess the possible hybridization of bees. Formed arrays of five new standards can be used in subsequent studies as standards of comparison.

Key words: Morphometry of wings, classification of worker bees, discriminant analysis.

 

GALATYUK O. https://orcid.org/0000-0002-9720-0660


YAROVETS V. https://orcid.org/0000-0001-7083-8130


BABENKO V. https://orcid.org/0000-0002-4278-6473


CHEREVATOV V. https://orcid.org/0000-0003-4785-1913


GRIGORENKO A. https://orcid.org/0000-0003-4301-0674


STRILCHUK M. https://orcid.org/0000-0001-6799-073X


KRYVCHENKO O.


  1. Selektsiia ta rozvedennia bdzhil: navchalnyi posibnyk [Selection and breeding of bees: a study guide]. M. K. Bohdan, N. O. Kirovych, V. M. Yasʹko, S. O. Petrenko, YE. O. Kotlyar. Kyiv.: CONDOR Publishing House, 2018. – 228 p.
  2. Apiculture. / Cherkasova, A. I. etc.; under the editorship A. I. Cherkasova. K: Harvest, 1989. 304 p. (in Ukrainian).
  3. Polishchuk, V. P. (2001). Apiculture. K: Ukrainian Beekeeper Publishing House, 296 p. (in Ukrainian).
  4. Polishchuk, V.P. (2006). Intrabreed type of Ukrainian bees "Khmelnytskyi". K: Beekeeper, no. 1, pp. 12–13. (in Ukrainian).
  5. Polishchuk, V. P. (2007). Selection of Ukrainian bees. K: Apiary, no. 11, pp. 2–5. (in Ukrainian).
  6. Hryhorkiv, L. M. (2018). Comparative assessment of the quality of bees from queens of different generations of genealogical groups. K: Beekeeping of Ukraine, Vol. 1, no. 3, pp. 29–35. Available at:https:// www.journalbeekeeping.com.ua/index. php/1_4/article/view/107 (in Ukrainian).
  7. Ruttner, F. (1988). Zuchttechnik und Zuchtauslesebei der Biene. Ehrenwirth-Verlag. Germani, 138 p.
  8. Podolsky M. S., Burenin M. L., Kotova H. M. Promyslove bdzhilnytstvo [Industrial beekeeping]. Kyiv.: Higher School, 1988. 336 p.
  9. Engel, Machael S. (1999). The Taxonomy of Recent and Fossil HoneyBees. Journal of Hymenoptera Research. Vol. 8 (2), pp. 165–196. ISSN 1070-9428. Archived from the original on March 15, 2017.
  10. Meixner, M. D., Worobik, M., Wilde, J., Fuchs, S., Koenigen, N. (2007). Apis mellifera mellifera in eastern Europe - morphometric variation and determination of its range limits. Apidologie, Springer Verlag. Vol. 38(2), pp. 191–197. Available at:https:// hal.archives-ouvertes.fr/hal-00892245.
  11. DAWINO (Discriminant Analysis With Numerical Output). Morphometrical analysis and taxonometrical classification of honey bees by wing characters. Available at:http://beedol.cz/dawino/DAWINO_prosp_EN.pdf.
  12. Tofilski, A. (2008). Using geometric morphometrics and standard morphometry to discriminate three honey beesub species. Apidologie. Vol. 39, pp. 558–563.
  13. Papp, V. V., Kerek, S. S. (2015). Study of breed characteristics of Carpathian bees using the "Beemorph" software. K: Beekeeping of Ukraine, no. 1, pp. 103–109. (in Ukrainian).
  14. Cherevatov, O. V., Panchuk, I. I., Kerek, S. S., Volkov, R. A. (2019). Molecular diversity of the CoICoII spacer region in the mitochondrial genome and the origin of the Carpathian bee. Cytology and Genetics. no. 4, pp. 13–14. DOI:10.3103/S0095452719040030.
  15. Galatiuk O. Ye. Zberezhennia ukrainskoi stepovoi porody bdzhil [Preservation of the Ukrainian steppe breed of bees]. URL: https://kermanych90.wixsite. com/mysite-1/single-post/2018/03/21/збереженняукраїнської-степової-породи-бджіл.
  16. Polishchuk V. P. Bdzhilnytstvo [Beekeeping]. Editorial office of the magazine "Ukrainian beekeeper". Lviv. 2001. 296 p.
  17. Yarovets, V., Babenko, V., Kryvchenko, O. (2022). Morphometry of the wings of bees in the central and eastern regions of Ukraine. K: Apiary, no. 2, pp. 22–25. (in Ukrainian).
  18. Tps Dig. Available at:https://ru.freedownloadmanager.org/Windows-PC/tps Dig2.html.
  19. Yerina, A.M., Yerin, D.L. (2014). Statistical modeling and forecasting: a textbook. K: KNEU, 348 p. (in Ukrainian).
  20. Yarovets, V. I., Babenko, V. V., Galatiuk, O. E. (2022). Morphometry of bee wings according to eight signs (indexes): Ci, Dbi, Disc.sh, Pci, Ri, Ci.2, Ci.2.1, Ci.3. K: Beekeeping of Ukraine, Vol. 1, no. 8, pp. 65– 71. DOI:10.46913/beekeeping journal.2022.8.10 (in Ukrainian).
  21. DSTU 4985:2008. Bee families of the Ukrainian steppe breed. Specifications. 2. Requirements of the standard for morpho-ethological features of bees. [Effective from 2010-01-01]. Kind. officer Kyiv: 2008, 13 p. (in Ukrainian).
  22. Yarovets, V., Babenko, V., Mozharovskyi, I., Sakara, P. (2021). Wing morphometry of some lines of the "Caucasian Mountain Gray" breed. Lviv: Ukrainian beekeeper, no. 6, 4 p. (in Ukrainian).
  23. Ruttner, F., Tassencourt, L., Louveaux, J. (1978). Biometrical-statistical analysis of the geographic variability of Apis mellifera L. Apidologie. Vol. 9(4), pp. 363–381. DOI:10.1051/apido:19780408.
  24. Yarovets, V., Babenko, V., Fertsak, M., Shved, O. (2023). Morphometry of the wings of worker bees of the Carpathian region (Strysky district, Skolivska hromad). K: Apiary, no. 1, pp. 21–23. (in Ukrainian).
  25. Lipatov, V., Yarovets, V. (2023). Morphometric standards of wings of worker bees of the subspecies A.m.carnica. K: Pasika, no. 3, pp. 16–18. (in Ukrainian).

 

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BTF №1 2023