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Biochemical parameters and productive qualities of hens when using feed additive of cerium dioxide nanoparticles

In the modern poultry industry one of the urgent problems is the activation of adaptive capacity birds to enhance their productivity and saving. At the beginning of the XXI century broad prospects for obtaining adaptogenic and antioxidant drugs with unique properties of nanotechnology are shown. During long time lanthanoids are used to improve crop and livestock productivity, among them compounds of lanthanum, cerium and others. These elements with atomic number of 57 (LA – lanthanum) to 71 (Lu – lutetium) are in the third group of the periodic table of elements. To increase the productivity of livestock and poultry there are used complex products containing multiple lanthanide (LA, Ce, etc.). The effectiveness of their using is proved by different authors. New prospects of using of lanthanides as a means of improving the biological functions of the body are opened by nanotechnology. It is shown that the cerium dioxide transition in nanocrystalline state increases its biological activity and to optimize character of intracellular reactions due to inactivation of reactive oxygen in cells. The current strategy to combat oxidative stress involves the introduction of exogenous antioxidant agents that have the ability to recyclization (regeneration) of antioxidant properties and prolonged action. Nanocrystalline ceria (NDC) is an unique multifunctional material. It's using is much promising in many scientific fields and is associated with complex special physical and chemical properties. Those properties include oxygen non-stoichiometry, powerful antioxidant properties as well as the dependence of the obtained effects of particle size. The purpose of research is to study the effect of nanocrystalline cerium dioxide on metabolic parameters, performance and safety of egg birds. Scientific and economic research conducted on chicken laying cross-laying hen «Lohmann Brown» NNDTS Bila Tserkva National Agrarian University. For the experiment there were formed two unique groups of 50 heads of chickens aged 150 days on the basis of analogs. Chicken Laying hens of control group received basic diet (BR), according to the given recipe, which ensured their need for protein, energy and micronutrients and macronutrients with regard to age, live weight and direction of performance. During 20 weeks experimental group got a SRC drug with water (particle size is 1 <n <5 nm and it is stabilized by citrate shell) at a dose of 8.6 mg per liter for 14 days. After 7–10 day break course is repeated.

Studies indicate that blood biochemical parameters in chicken laying hens were within the physiological norm during the experiment. In the study of indicators possible differences between groups were not found, but throughout the experiment tend to increase of total protein, total lipids and calcium chickens in the experimental group was studied. The difference between the rates was not significant. It was found that chickens in experimental group had a trend to reduced activity of ASAT and ALAT in blood. This change of aminotransferase activity of animal blood in the experimental group can be attributed to some hepatoprotective effect of cerium dioxide nanoparticles founded in other studies. The levels of creatinine, uric acid, cholesterol and phosphorus in the blood of the birds of research groups under influence of the cerium dioxide nanoparticles were not significantly different from controls. It is established that the watering of chickens-laying hens with nanocerium at a dose of 8.6 mg/L positively affects for their egg productivity. Studies indicate that in all periods of chicken laying hens in research experimental group exceeded the performance of hens in control group. Using in the experimental group of nanocrystalline cerium dioxide promoted receivable 132.1 eggs per primary chicken laying hen during the whole period of the experiment and 122.2 eggs per chicken laying hen per control groop. Safety of chickens in the experimental group was 98 % and 94 % in control group. Number of egg masses which was obtained in laying hen chickens in the experimental group was 7.5 % higher than in the control group.

Thus it was established that the feed additive "nanocerium" in feeding laying hens provides a higher level of performance relative to controls, while it is not show adverse effect on product quality eggs. Results of conducted atomic emission analysis show that the applied dose of nanocrystalline cerium dioxide is not accumulated in protein, yolk, shell eggs, lungs, liver, kidneys and oviduct of laying hens and it was not higher than background values in control group. Thus there was established a positive effect on the studied additives egg performance of chicken laying hens in the experiment. There weke no admitted negative effects on biochemical blood parameters, egg quality product. In the applied dose of nanocrystalline cerium dioxide did not accumulate in the eggs and poultry parenchymal organs.

Key words: poultry, antioxidants, nanotechnologies, cerium dioxide, chicken laying hens, biochemical characteristics, egg production.

U. Shadura


M. Spivac


BITYUTSKII V. https://orcid.org/0000-0002-2699-3974


MELNICHENKO O. https://orcid.org/0000-0001-5462-508X


I. Sotnichenko


O. Shcherbakov


DEMCHENKO O. https://orcid.org/0000-0003-1457-143X


N. Zholobak


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2015
BTF №2 2015