You are here

Characteristics of the genetic structure of the β-casein gene of producers approved for use in Ukraine in 2020

The modern topical issue in dairy cattle breeding is the study of the relationship between hereditary factors that determine the types of proteins in milk. Casein is the main component of milk proteins and is represented by three fractions - alpha (CSN1S1), beta (CSN2) and capa (CSN3). The content of individual casein fractions depends on the breed of cows. Scientists point out some grand questions as for the promotion of milk proteins, like beta-casein, affecting on such disorders as type-1 diabetes, schizophrenia, autism and the sudden death of an infant. The economic component that will ensure the attractiveness of creating the dairy herds completed with animals of genotype А2 А2 is the higher price of raw milk in comparison with conventional milk. The aim of this work is to evaluate the genotype of bulls-producers by the beta-casein gene, which are allowed to be used in Ukrainian farms in 2020. It is established that the majority of such bulls-producers are evaluated by the genotype of betta-casein. The largest proportion of bulls with the desired A2A2 genotype was found in red Danish and red Norwegian bulls. A greater proportion of A1A1 genotypes are characterized by Holstein bulls of red-mottled color. The largest proportion of the desired A2 allele was found in bulls of red Danish, red Norwegian and Guernsay breeds. Producers of Holstein and Ayshire breeds were characterized by a smaller proportion of this allele. It was found that the use of sperm of bulls approved for use in 2020 with the A2A2 by β-casein genotype on the breeding stock of domestic breeds will improve the economically useful characteristics of offspring, and will contribute the desired genotype of cows. As a result of the analysis of the genotype of Holstein and Swiss breeds that were used in breeding farms of Sumy region over the past three years, we found that 38 Holstein bulls were used in breeding farms and in breeding plants, among which 11% were evaluated by the β-casein gene. Among 12 Shvits bulls that were used in farms of the region, only 42% were evaluated by the β-casein genotype. This confirms that the work with the formation of dairy herds completed with animals of genotype А2 А2 in Sumy region was not carried out.

Key words: breed, bull, β-casein, genotype, allele, daughters milk productivity.

  1. Marchi De, M., Dal Zotto, R., Cassandro, M., Bittante, G. (2007). Milk Coagulation Ability of Five Dairy Cattle Breeds. Journal of Dairy Science. Vol. 90, no. 8, pp. 3986– 3992. Available at:https://doi.org/10.3168/jds.2006-627
  2. Il`ina, A. (2014) Geneticheskaya oczenka sostoyaniya populyaczionnogo genofonda krupnogo rogatogo skotaYaroslavskoj porody` v OAO «Mikhajlovskoe» Yaroslavskogo rajona [Genetic assessment of the population of the gene pool of cattle of the Yaroslavl breed in OAOMikhailovskoye of the Yaroslavl region]. Vesnik APK Verkhnevolzh`ya. [Bulletin of the AIC of the Upper Volga]. no. 4 (28), pp. 39–43. Available at:http://www.yaragrovuz. ru/images/Vestnik_APK/14-4/4-2014_39-43.pdf
  3. Khlestkina, E. (2013). Molekulyarny`e markery` v geneticheskikh issledovaniyakh i v selekczii [Molecular markers in genetic research and in breeding] Vavilovskij zhurnal genetiki i selekczii [Vavilov Journal of Genetics and Breeding]. Vol. 17, no. 4/2, pp. 1044–1054. Available at:http:// www.bionet.nsc.ru/vogis/download/17-4/2/21Khlestkina.pdf
  4. Islam, M., Alam, M., Islam, M., Khan, M., Ekeberg, D., Rukke, E., Vegarud, G. (2014). Principal Milk Components in Buff alo, Holstein Cross Indigenous Cattle and Red Chittagong Cattle from Bangladesh Islametal. Asian Australas Journal Animal Science. Vol. 27, pp. 886–897.
  5. Elliott, R., Harris, D., Hill, J., Bibby, N., (1999) Wasmuth H.E.Type I (insulin-dependent) diabetes me llitus and cow milk: casein variant consumption Diabetologia. Vol. 42, pp. 292–296. Available at:https://doi.org/10,1007 / s001250051153
  6. Kučerova, J., Matějiček, A., Jandurova, O., Sorensen, P., Němcova, E, Štipkova, M., Kott, T., Bouška, J., Frelich, J. (2006). Milk protein genes CSN1S1, CSN2, CSN3, LGB and their relation to genetic values of milk production parameters in Czech Fleckvieh. Czech Journal Animals. Science. Vol. 51(6), рp. 241–247. Available at:https://www. agriculturejournals.cz/publicFiles/52288.pdf
  7. Drozdov, E., Zayakin, V., Nam, I. (2009). Analysis of polymorphism of genes for kappa-casein, β-lactoglobulin, prolactin, gene for releasing factor and growth hormone by alui and mspi markers in Ayrshire cows [Analysis of polymorphism of the genes of kappa-casein, β-lactoglobulin, prolactin, the gene for releasing factor and growth hormone by alui and mspi markers in cows of Ayrshire breed]. Bulletin of the Bryansk State University. [Bulletin of the Bryansk State University]. no. 4, pp. 152–156. Available at:https:// cyberleninka. Ru / article / n / analiz-polimorfi zma-genov-kappa-kazeina-laktoglobulinaprolaktina-gen-rilizing-faktora-i-somatotropina-po-alui-imspi-markeram-u-korov/ viewer
  8. Kyselová, J., Ječmínková, K., Matějíčková, J., Hanuš, O., Kott, T., Štípková, M., Krejčová, M. (2019). Physiochemical characteristic sand fermentation ability of milk from Czech Fleck vieh cow sarerelated to genetic polymorphisms of β-casein, κ-casein, and β-lactoglobulin. Asian-Australas J AnimSci. Vol. 32, pp.14–22. Available at:https://doi.org/10.5713/ajas.17.0924
  9. Kovalyuk, N., Saczuk, V., Kovalyuk, M., Machul`skaya, E. (2019). Selekcziya krupnogo rogatogo skota po polimorfnomu genu beta-kazeina v Krasnodarskom Krae [Cattle selection by the polymorphic beta casein gene in the Krasnodar Territory]. Genetika i razvedenie zhivotny`kh [Genetics and animal breeding]. no.1, pp. 22–24. Available at:http://vniigen.ru/zhurnal-1-2019-kovalyuk-n-v/
  10. Kononova, L., Sy`chova, O. (2016). Neoby`knovennoe korov`e moloko [Unusual cow's milk]. Molochnaya reka [Milk river]. no. 3(63), pp. 62–64.
  11. Kuz`menko, N., Kuzina, A. (2016). Rol` beta-kazeina v pitaniidetej pervy`kh let zhizni [The role of beta-casein in the diet of the fi rst years of life]. Lechashhij vrach [Attending doctor]. no. 01(16), pp.75–80.
  12. Trukhachev, V.I., Olejnik, S.A., Zly`dnev, N.Z. (2017) Metodicheskie rekomendaczii po sozdaniyu molochny`kh stad krupnogo rogatogo skota s uluchshenny`mi pokazatelyami po soderzhaniyu belka v moloke na osnove allel`ny`kh variantov frakczij kazeina: rekomendaczii dlya zooveterinarnikh speczialistov [Guidelines for the creation of dairy herds of cattle with improved indicators for the protein content in milk based on allelic variants of casein fractions: recommendations for veterinarian specialists]. Stavropol. 97 p.
  13. Czarenko, O.M., Zlobi`n, Yu.A., Sklyar, V.G., Panchenko, S.M. (2000). Komp'yuterni` metodi v si`l`s`komu gospodarstvi` ta bi`ologi`yi: Navchal`nij posi`bnik [Computer Methods in Agriculture and Biology: A Tutorial]. Sumi. 203 p.
  14. Kovalyuk, N., Saczuk, V., Machul`skaya, E. Shakhnazarova, Yu. (2018) Perspektivy` ispol`zovaniya polimorfi zma gena β-kazeina v selekczii krupnogo rogatogo skota molochnogo napravleniya produktivnosti [Prospects for the use of β-casein gene polymorphism in breeding dairy cattle]. Molochnoe i myasnoe skotovodstvo [Dairy and beef cattle breeding]. no. 5, pp.14–16.
  15. Parashar , A., Saini, R. (2015). A1 milk and its controversy-a review. International Journal of Bioassays. Vol. 4.(12), pp. 4611–4619.Available at:https://doi.org/10.21746/ ijbio.2015.12.007
  16. Gradinaru, A., Petrescu-Mag, I., Oroian, F., Balint, C., Oltean, I. (2018). Milk Protein Polymorphism Characterization: a Modern Tool for Sustainable Conservation of Endangered Romanian Cattle Breeds in the Context of Traditional. Sustainability. Vol. 10, Issue 2(534), рp. 2–23. Available at:https://doi.org/10.3390/su10020534
  17. Barany, M., Aosze, Zs., Buchberger, J., Krause, I. (2019). Genetic Polymorphism of Milk Proteins in Hungarian Spotted and Hungarian Grey Cattle: A Possible New Genetic Variant of, β-Lactoglobulin. Journal of Dairy Science. Vol. 76 (2), рp. 630-635. Available at: https://doi.org/10.3168/jds. S0022-0302(93)77384-1
  18. Cieslinska, A., Fiedorowicz, E., Zwierzchowski, G., Kordulewska, N., Jarmołowska, B., Kostyra, E. (2019). This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Genetic Polymorphism of β-Casein Gene in Polish Red Cattle-Preliminary Study of A1 and A2 Frequency in Genetic Conservation Herd. Animals. no. 9, pp. 377–381. Available at: https://doi.org/10.3390/ani9060377
  19. Bobe, G., Lindberg, G., Freeman, A., Beitz, D. (2007) Short Communication: Composition of Milk Proteinand MilkFatty Acids is Stablef or Cows Differingin Genetic Meritfor Milk Production. Journal of Dairy Science. Vol. 90, no. 8, pp. 3955–3960. Available at: https://doi.org/10.3168/jds.2007-0099
  20. Marchi De, M., Dal Zotto, R., Cassandro, M., Bittante, G. (2007). Milk Coagulation Ability of Five Dairy Cattle Breeds. Journal of Dairy Science. Vol. 90, no. 8, pp. 3986– 3992. Available at: https://doi.org/10.3168/jds.2006-627
  21. Bonfatti, V., Di Martino, G., Cecchinato, A., Vicario, D., Carnier, P.(2010). Effects of β-κ-casein (CSN2- CSN3) haplo types and β-lactoglobulin (BLG) genotypes on milk production traits and detailed protein composition of individual milk of Simmental cows. Journal of Dairy Science. Vol. 93, no. 8, pp. 3797–3808. Available at:https:// doi.org/10.3168/jds.2009-2778/
AttachmentSize
PDF icon ladyka_pavlenko_1_2020.pdf1.93 MB