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The cell protection of weated pigs for probiotics

The immune system is central to ensuring the consistency of the body's homeostasis. The state of the body's natural resistance is determined by a set of non-specific protective mechanisms. Lymphocytes and phagocytes are actively involved in maintaining immunity. Lymphocytes recognize the antigens of pathogenic microorganisms, and phagocytes absorb and destroy the pathogens themselves. During the weaning of piglets from sows there is a decrease in the protective forces of their body. During this period, the natural resistance of the piglets is reduced due to the stressful situation caused by changing conditions of confinement, the transition to full feed and lack of sows. The immune system of weaning pigs is relatively weak, so when exposed to environmental and technological stressors, they become susceptible to various diseases. The use of probiotic drugs stimulates the activity of the immune system, prevents stress and immunodeficiency. One of these probiotics is the probiotic of domestic production Protecto-active. It w observed the the influence of the probiotic Protecto-active on the indices of nonspecific resistance of the young pigs organism to the growth. An increase in bactericidal activity of blood serum by 12.10% (P <0.05) and lysozyme activity of blood in the piglets of the experimental group was increased by 3.71% compared to control, which indicates the activation of the body's defenses and the increase in adaptive capacity. An important step in the study of the influence of the probiotic Protekto-active on the state of the immune system is to determine the phagocytic activity of neutrophils, phagocytic index and phagocytic number. In the experimental group of piglets that were fed the probiotic Protecto-active, we found an increase in leukocyte phagocytic activity by 9.0% (P <0.001), a phagocytic index by 51.7% (P<0.001) and a phagocytic number by 24.8% ( P <0.01) compared with the control group. Thus, using a probiotic Protecto-active, all indicators of phagocytosis increase: the number of phagocytes increases, their ability to capture microorganisms and increases their digestive capacity, it increases the bacterial and lysozyme activity of blood serum, which is positively reflected in the immunobiosity. Key words: probiotic, phagocytosis, phagocytic index, phagocytic number, phagocytic activity of leukocytes, cellular immunity, piglets

BONDARENKO L. https://orcid.org/0000-0003-3751-9140


  1. Isaacson, R. H., Kim, B., Isaacson, R. (2012). The intestinal microbiome of the pig. Animal Health Research Reviews. Vol. 13 (1), pp. 100–109.
  2. Potočnjak, D., Kezić, D., Popović, M. (2012). Agerelated changes in porcine humoral and cellular immune parameters. Veterinarski arhiv. Vol. 82 (2), pp. 167–181.
  3. Masljanko, R.P., Bozhik, N.D. (2014). Levkіvs'ka Rol' apoptozu v іmunnih reakcіjah organіzmu [The role of apoptosis in the body's immune responses]. Naukovij vіsnik L'vіvs'kogo nacіonal'nogo unіversitetu veterinarnoї medicini ta bіotehnologіj іmenі S.Z. Gzhic'kogo [Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv]. Vol. 16, no. 2 (59), Part 2, pp. 225–230. 65
  4. Trevisi, P., Pérez, J.F. (2017). Diets and pig gut health: preface. Animal Feed Science and Technology. Vol. 233, pp. 87–88.
  5. Іmunіtet porosjat na maksimum [Piglets' immunity is at its maximum]. Pributkove svinarstvo [Profits pig farming]. Vseukrains'kij zhurnal [All-Ukrainian magazine]. 2012, no. 6 (12). Available at:http://profisvine.pigua.info/indexukr. php?id=102
  6. Lukashhuk, B.O., Slіvіns'ka, L.G. (2015). Vpliv fіtobіotika na pokazniki nespecifіchnoї rezistentnostі porosjat u pіdsisnij perіod [Influence of phytobiotic on indicators of nonspecific resistance of piglets in the suckling period]. Nauk. vіsnik L'vіv. nac. un-tu vet. medicini ta bіotehnologіj іmenі S.Z. Ґzhic'kogo [Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv]. Lviv, Vol. 17, no. 1 (61), Part 1, pp. 96–100.
  7. Lukashhuk, B.O. (2015). Pokazniki nespecifіchnoї rezistentnostі pіdsisnih porosjat za zgodovuvannja fіtobіotika [Indicators of nonspecific resistance of suckling piglets for feeding phytobiotics]. Naukovі poshuki molodі u tret'omu tisjacholіttі: tezi dopovіdej. Suchasnі problemi veterinarnoї medicini: Mіzhnar. nauk.-prakt. konf. molodih uchenih, aspіrantіv і doktorantіv. (14–15 travnja 2015 r.) [Scientific research of young people in the third millennium: abstracts of reports. Modern problems of veterinary medicine: International scientific-practical conf. young scientists, graduate students and doctoral students. (May 14-15, 2015)]. Bіla Tserkva, 13 p.
  8. Pohil'ko, Ju.M., Kravchenko, N.O. (2016). Vpliv seredovishha kul'tivuvannja na antagonіstichnu aktivnіst' molochnokislih bakterіj [The influence of the culture medium on the antagonistic activity of lactic acid bacteria]. Sіl's'kogospodars'ka mіkrobіologіja [Agricultural microbiology]. Issue 24, pp. 64–72.
  9. Merlіch, A.G., Lіmans'ka, N.V. (2016). Antagonіstichna aktivnіst' bakterіj Lactobacillus plantarum, vidіlenih z roslinnih dzherel Ukraіni ta Francії, proti fіtopatogennih bakterіj [Antagonistic activity of Lactobacillus plantarum bacteria isolated from plant sources of Ukraine and France against phytopathogenic bacteria]. Mіkrobіologіja і bіotehnologіja [Microbiology and biotechnology]. no. 4, pp. 71–85.
  10. Skibіc'kij, V.G., Kozlovs'ka, G.V., Іbatullіna, F.Zh. (2013). Metodichnі rekomendacії z konstrujuvannja probіotikіv ta zastosuvannja їh u prakticі veterinarnoї medicini [Methodical recommendations for the design of probiotics and their application in the practice of veterinary medicine]. K.: CJSC "Nichlava", 39 p.
  11. Kovalenko, V.F. (2010). Vpliv okremih mіkrobіotichnih preparatіv na procesi travlennja [Influence of separate microbiotic preparations on digestive processes]. Veterinarna medicina Ukraini [Veterinary medicine of Ukraine]. no. 13, 58 p.
  12. Arena, M.P., Capozzi, V., Spano, G., Fiocco, D. (2017). The potential of lactic acid bacteria to colonizebiotic and abiotic surfaces and the investigation of their interactions and mechanisms. Applied Microbiology and Biotechnology. 101(7), pp. 2641–2657.
  13. Chiu, Y.H., Lin, S.L., Tsai, J.J., Lin, M.Y. (2014). Probiotic actions on diseases: implications for therapeutic treatments. Food Funct. 5(4), pp. 625–634.
  14. Hill, C., Guarner, F., Reid, G. (2014). Expert consensus document: The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology and Hepatology. 11(8), pp. 506–514.
  15. Shirobokov, V. P., Jankovs'kij, D. S., Diment, G. S. (2010). Novі strategіі v oblastі stvorennja і klіnіchnogo vikoristannja probіotikіv [New strategies in the field of creation and clinical use of probiotics]. Vіsn. farmakologіі ta farmacіі [Bulletin of Pharmacology and Pharmacy]. no. 2, pp. 18–30.
  16. Batista, A., Silva, M., Raices, R. (2015). Quality parameters of probiotic yogurt added to glucose oxidase compared to commercial products through microbiological, physical–chemical and metabolic activity analyses. Food Res. Int. Vol. 77, pp. 627–635.
  17. Kanmani, P., Satish, Kumar R., Yuvaraj, N. (2013). Probiotics and its functionally valuable products – A review. Crit. Rev. Food Sci. Nutr. Vol. 53, no. 6, pp. 641–658.
  18. Papadimitriou, K., Zoumpopoulou, G., Foligne, B. (2015). Discovering probiotic microorganisms: in vitro, in vivo, genetic and omics approaches. Frontiers in Microbiology. Vol. 6, 58 p.
  19. Mokrozub, V. V., Lazarenko, L. M., Babenko, L. P. (2012). Antibakterіal'nі j іmunomoduljuval'nі vlastivostі shtamіv lakto- ta bіfіdobakterіj za eksperimental'noi stafіlokokovoi іnfekcіi [Antibacterial and immunomodulatory properties of lacto- and bifidobacterial strains in experimental staphylococcal infection]. Bіotehnologіja [Biotechnology]. Vol. 5, no. 2, pp. 98–104.
  20. Maljar, D.D., Mel'nichenko, Ju.O., Solomonjuk, Ja.V., Bіtjuc'kij, V.S. (2013). Vivchennja efektivnostі zastosuvannja probіotikіv ta prebіotikіv na іmunologіchnі ta mіkrobіologіchnі pokazniki perepelіv [Study of the effectiveness of probiotics and prebiotics on immunological and microbiological parameters of quails]. Tehnologіja virobnictva і pererobki produkcії tvarinnictva: zb. nauk. prac' [Technology of production and processing of livestock products: a collection of scientific papers]. Bіla Tserkva, Issue 10 (105), pp. 53–56.
  21. Mel'nichenko, Ju.O., Maljar, D.D., Lazarenko, L.M. (2014). Doslіdzhennja іmunomoduljuval'noі dіі novih probіotichnih preparatіv [Investigation of the immunomodulatory effect of new probiotic drugs]. Nauk.- tehn. bjul. Іn-tu bіologіі tvarin; DNDKІ vetpreparatіv ta kormovih dobavok [Scientific and technical byul. Inst. Of Animal Biology; DNDKI veterinary drugs and feed additives]. Issue 15, no. 1, pp. 201–208.
  22. Zhila, M. І., Levic'kij, T. R., Kushnіr, І. M. (2014). Farmakologіchnі vlastivostі probіotichnih kormovih dobavok ta їh vpliv na produktivnіst' porosjat pri vіdgodіvlі [Pharmacological properties of probiotic feed additives and their effect on the productivity of piglets during fattening]. Naukovo-tehnіchnij bjuleten' Іnstitutu bіologії tvarin, DNDKІ vetpreparatіv ta kormovih dobavok [Scientific and technical bulletin of the Institute of Animal Biology, DNDKI veterinary drugs and feed additives]. Lvіv, Issue 15, no. 1, pp. 158–163.
  23. Stojanovs'kij, V.G. (2011). Probіotiki ta іmunna sistema [Probiotics and the immune system]. Veterinarіja [Veterinary medicine]. no. 4 (101), pp. 21–25.
  24. Hizhnjak, O.S., Krasnopol's'kij, Ju.M. (2013). Bіotehnologіchnі aspekti otrimannja kompleksnogo preparatu, jakij mіstit' rіznі shtami probіotichnih kul'tur [Biotechnological aspects of obtaining a complex preparation containing different strains of probiotic cultures]. Vіsnik NTU «HPІ» [Bulletin of NTU "KhPI"]. Kharkiv: NTU "KhPI", no. (978), pp. 113–118.
  25. Hizhnjak, O.S., Krasnopol's'kij, Ju.M. (2012). Bіotehnologіchnі aspekti stvorennja preparatіv na osnovі probіotikіv [Biotechnological aspects of creating drugs based on probiotics]. Vіsnik NTU «HPІ» [Bulletin of NTU "KhPI"]. Kharkiv: NTU "KhPI", no. 44 (950), pp. 72–78.
  26. Zhemerova, K.G., Dunaj, O.V., Galkin, O.Ju., Malder, L., Hemert, S.van. (2012). Rozrobka metodіv kontrolju jakostі ta doslіdzhennja probіotichnogo preparatu, priznachenogo dlja lіkuvannja і profіlaktiki alergіi ta disbakterіozu [Development of methods of quality control and research of a probiotic drug intended for the treatment and prevention of allergies and dysbacteriosis]. Ukrains'kij zhurnal klinichnoi ta laboratornoї medicini [Ukrainian Journal of Clinical and Laboratory Medicine]. Vol. 7, no. 4, pp. 169–174.
  27. Starovojtova, S.O., Skroc'ka, O.І., Penchuk, Ju.M., Pirog, T.P. (2012). Tehnologіja probіotikіv [Technology of probiotics]. Pіdruchnik [Textbook K .: NUHT, 318 p.
  28. Cherniavskyi, O., Babenko, S., Bomko, V., Dyachenko, L., Slomchynskyi, M., Chernyuk, S., Kuzmenko, O., Tytariova, O., Horchanok, A., Polishchuk, V., Bilkevych, V., Polishchuk, S., Ponomarenko, N. (2019). Productivity and mineral exchange in the body of young pigs when feeding probiotics. Ukrainian Journal of Ecology. 9 (1), pp. 220–225.
  29. Kuzmenko, O., Bomko, V., Babenko, S., Horchanok, A., Slomchinskyy, M., Tytariova, O., Chernyavskyy, O., Prisjazhnjuk, N. (2018). Influence of mannan oligosaccharides for getting high quality ecologically safe swine production. Ukrainian Journal of Ecology. 8 (2), pp. 225–229. Available at:https/doi.org/10.15421/2018_331.
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2020
BTF №2 2020.