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PEROXIDATION PROCESSES IN THE RABBIT ORGANISM DURING POSTNATAL ONTOGENESIS

One of the pressing problems of modern biochemistry is the problem of adaptation of animal organism to the environment and the formation of an adaptive reaction to the negative impact of production stress factors. Among such adaptive mechanisms for rabbits in the conditions of intensive rabbit meat management is the development of oxidative stress, which causes the accumulation of reactive oxygen species in the body and the development of reactive oxygen pathology. An important role in the mechanism of adaptation of the body belongs to lipids, because they are a structural component of cell membranes and act as energy and signal systems in cells. Peroxide oxidation of lipids is a compensatory reaction that ensures the functioning of the organism for changes in the environment. The content of total lipids and peroxide oxidation products of lipids, as well as the activity of enzymes of the antioxidant defense system in rabbits from birth to 90 days of age was investigated. It has been established that the content of total lipids in brain tissues increases throughout the period of postnatal ontogenesis due to the peculiarities of the functional and metabolic activity of brain cells. The content of common lipids is closely related to the processes of lipid peroxide oxidation and the activity of enzymes of antioxidant defense. The growth in concentration of peroxide oxidation products is accompanied by a decrease in the content of total lipids in the rabbit tissues. Reduced content of TBARSproducts in rabbit brain tissue from birth to 90-day age was noted. A moderate (r = 0.66) correlation between the content of lipid conjugated dienes and lipid hydroperoxides, as well as the strong correlation (r = -0.77) between the contents of lipid conjugated dienes and TBARS-products has been established. In the heart of rabbits a reversible moderate (r = -0.62) correlation between the content of lipid conjugated dienes and lipid hydroperoxides has been revealed.

Key words: rabbits, development, lipid peroxidation, brain, heart, longest muscle of the back.

ROL N. https://orcid.org/0000-0003-0295-4193


TSEKHMISTRENKO S. https://orcid.org/0000-0002-7813-6798


VOVKOHON A. https://orcid.org/0000-0002-0521-2737


POLISHCHUK V. https://orcid.org/0000-0002-0602-6100


POLISHCHUK S. https://orcid.org/0000-0001-6716-848X


PONOMARENKO N.


FEDORCHENKO M.


  1. Hilmutdino va, M.Sh., Cherno, V.S., Kosharnyi, V.V. (2018). Riven pervynnykh produktiv peroksydnoho okysnennia lipidiv za umov riznomanitnoi funktsionalnoi aktyvnosti skeletnykh miaziv v kombinatsii zi zminamy fotoperiodu [The level of primary products of lipid peroxidation under various functional activity of the skeletal muscle in combination with changes in the photoperiod]. Svit medytsyny ta biolohii [The world of medicine and biology]. no. 3, pp. 215–218. Available at:https://doi.org/10.26724/2079-8334-2018-3-65- 215-218
  2. Kolesnikova , L.I., Darenskaya, M.A., Rashidova, M.A., Sholokhov, I., Grebenkina, L.A., Vanteeva, O.A. (2016). Lipid Peroxidation State in Women of Reproductive Age with Acute Form of Viral Hepatitis. Bull. of the Russian Academy of Medical Sciences. Vol. 71 (1), pp. 11–15. Available at: https://doi.org/10.15690/vramn625
  3. Paienok, O.S ., Paienok, A.V., Pankiv, I.V., Kostiv, M.O. (2019). Zminy pokaznykiv peroksydatsii lipidiv ta antyoksydantnoi aktyvnosti u vahitnykh ta rodil iz dyfuznym toksychnym zobom [Winter indicators of peroxidation of lipid and antioxidant activity in children and children with toxic toxic goiter]. Svit medytsyny ta biolohii [Light Medicine and Biology]. no. 2 (68), pp. 85–91. Available at:https://doi.org/10.26724/2079-8334- 2019-2-68-85-91
  4. Polishchuk S. A., Chornozub T.V., Tsekhmistrenko S.I. (2011). Vmist TBK-aktyvnykh produktiv ta aktyvnist fermentiv antyoksydantnoi systemy u plazmi spermy knurivplidnykiv z pokaznykamy normalnoi i nyzkoi yakosti [The range of TAC-active products and the activity of antioxidant system enzymes in plasma sperm are normal and with indicators of normal and low viscosity]. Nauk. visn. vet. Med [Science News of Veterinary Medicine]. Bila Tserkva, Issue 8 (82), pp. 179–182. Available at:http://rep.btsau.edu.ua/ handle/BNAU/2145
  5. Sushko, O.O., Iskra, R.Ja., Pryjmych, V.I. (2018). Vplyv tsytratu khromu na antyoksydantnyi zakhyst u pechintsi shchuriv z eksperymentalno indukovanym diabetom [Eff ect of chromium citrate on antioxidant protection in liver of rats with experimentally induced diabetes]. Biolohiia tvaryn [Animal biology]. Vol. 20, no. 4, pp. 61–68.
  6. Tarnovska, A.V ., Heneha, A.B., Semochko, O.M., Yaremchuk, M.M. (2018). Peroksydne okysnenia lipidiv u zarodkakh viuna protiahom rannoho embriohenezu za vplyvu antybiotyku ftorkhinolonovoho riadu borotsynu [Lipid oxidation peroxide in henna embryos during early embryogenesis due to antibiotic fl uoroquinolone series borocin]. Molodyi vchenyi [Young scientist]. no. 9, pp. 290–293.
  7. Tyshchenko, L., Shtonda, O., Pylypchuk, O., Menchynska, A., Shakhvorostova, V. (2019). Ptashyni zhyry [Bird fat]. Osoblyvosti skladu ta kharakterystyky yikhnoi stiikosti do okyslennia [Features of the composition and characteristics of their resistance to oxidation]. Food Science and Technology. no. 13 (4). Available at:https://doi. org/10.15673/fst.v13i4.1569
  8. Usenko, S.O., Sh ostia, A.M., Slynko, V.H., Bondarenko, O.M., Bereznytskyi, V.I., Shaferivskyi, B.S., Chukhlib, Ye.V. (2019). Osoblyvosti perebihu protsesiv peroksydnoho okysnennia u svynok zalezhno vid fi ziolohichnoho stanu [Features of the course of the processes of peroxide oxidation in pigs depending on the physiological state]. Visnyk PDAA [PDAA Bulletin]. no. 2, pp. 92–97.
  9. Chernushkin, B. O., Slivinska, L.H., Vlizlo, V.V. (2016). Pokaznyky peroksydnoho okysnennia lipidiv u krovi vivtsematok, khvorykh na hepatodystrofi iu [Indicators of lipid peroxidation in the blood of ewes suff ering from hepatodystrophy]. Naukoyvi visnyk LNU VMBT im. S.Z. Hzhytskoho [Scientifi c Bulletin Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv]. no. 18, pp. 202–206. Available at: https://doi.org/10.15421/ nvlvet6641
  10. Аmaral, A.B., da S ilva, M.V., Caetano, da Silva., Lannes, S. (2018). Lipid oxidation in meat: mechanisms and protective factors – a review. Food Sci. Technol, Campinas. Vol. 38, рр. 1–15. Available at:https://doi.org/10.1590/ fst.32518
  11. Dominguez-Perles, R. , Gil-Izquierdo, A., Ferreres, F., Medina, S. (2019). Update on oxidative stress and infl ammation in pregnant women, unborn children (nasciturus), and newborns - Nutritional and dietary eff ects. Free Radical Biology and Medicine. Vol. 142, pp. 38–51. Available at: https://doi.org/10.1016/j.freeradbiomed.2019.03.013.
  12. Duhig, K., Chappell, L.C., Shennan, A.H. 92016). Oxidative stress in pregnancy and reproduction. Obstetric Medicine. Vol. 9 (3), pp. 113–116. Available at: https://doi. org/10.1177/1753495x16648495.
  13. Jetty Chung-Yung, Lee. , Thierry, Durand. (2019). Lipid Peroxidation: Analysis and Applications in Biological Systems. Antioxidants Vol. 8, 40 p. Available at: https://doi. org/10.3390/antiox8020040
  14. Katerji, М., Filippova, M., Duerksen-Hughes, P. 2019. Approaches and Methods to Measure Oxidative Stress in Clinical Samples: Research Applications in the Cancer Field. Oxidative Medicine and Cellular Longevity. Vol. 2019, 29 p. Available at: https://doi.org/10.1155/2019/1279250
  15. Li, S., Tan, H.Y, Wang , N., Zhang, Z.J., Lao, L., Wong, C.W., Feng, Y. (2015). The Role of Oxidative Stress and Antioxidants in Liver Diseases. International Journal of Molecular Sciences. Vol. 16 (11), pp. 2608–2612. Available at: https://doi.org/10.3390/ijms161125942
  16. Romanova, L.A., Stal`na ya, I.D. (1977). Metod opredeleniya gidroperekisej lipidov s pomoshh`yu, tioczianata ammoniya [A method for determining lipid hydroperoxides using ammonium thiocinate]. Sovremenny`e metody` v biokhimii [Modern methods in biochemistry]. M.: Medicine, pp. 64–66.
  17. Su, L.J., Zhang, J.H., Gomez, H., Murugan, R., Hong, X., Xu, D., Jiang, F., Peng, Z.Y. (2019). Reactive Oxygen Species-Induced Lipid Peroxidation in Apoptosis, Autophagy, and Ferroptosis. Oxidative Medicine and Cellular Longevity. Vol. 2019, 13 p. Available at: https://doi. org/10.1155/2019/5080843
  18. Tsekhmistrenko, S., Rol, N., Fedorchenko, M. (2019). Peroxide oxidation processes and enzyme activity of the antioxidant system in the organism of rabbits of the New Zealand breed. Biologija. 65, pp. 12–19. Available at: https:// doi.org/10.6001/biologija.v65i1.3982
  19. Tsekhmistrenko, S.I., Bit yutskyy, V.S., Tsekhmistrenko, O.S., Polishchuk, V.M., Polishchuk, S.A., Ponomarenko, N.V., Melnychenko, Y.O., Spivak, M.Y. (2018). Enzyme-like activity of nanomaterials. Regulatory Mechanisms in Biosystems. 9(3), pp. 469–476. Available at: https://doi.org/10.15421/021870.
  20. Vlizlo, V.V., Fedoruk, R.S ., Ratych, I.B. (2012). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni [Laboratory research methods in biology, livestock and veterinary medicine]. Lviv, 762 p.
  21. Stal`naya, I.D. (1997). Metod opredeleniya dienovoj kon`yugaczii nenasy`shheny`kh vy`sshikh zhirny`kh kislot [Method for the determination of diene conjugation of unsaturated higher fatty acids]. Sovremenny`e metody` v biokhimii [Modern methods in biochemistry]. M.: Medicine, pp. 63–64.
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2020
BTF №1 2020